Part:BBa_K133009:Design

CF213-UB33-CF215-RGD-Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1376
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 718
  Illegal BamHI site found at 933
  Illegal BamHI site found at 1444
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1727
  

Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

plasmid DNA (CF213 [1] and CF215 [2] are deposited in the registry). UB33 was ordered from GeneArt.